ZFIN Image: Bu et al., 2021, Figure 4

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Figure 4

An F0 screen of 11 target genes in 7 known CM pathways identified mapk3 and mtor as 2 therapeutic modifier genes for VEC.

(A) Representative images of the heart area of F0 fish at 3 dpf. The black star indicates severe edema, and red stars indicate mild edema. Scale bar: 300 μm. (B) Fluorescence immunostaining images using anti-myosin heavy chain 1 (F59) in the ventricles of (from top to bottom): WT controls, vmhcl^e13/e13, vmhcl^e13/e13;mapk3^e2-MJ, and vmhcl^e13/e13;mtor^e6-MJ mutant hearts at 3 dpf. Scale bar: 2 μm; V, ventricle. (C) Kaplan–Meier survival curves of vmhcl^e13/e13 mutant fish upon mapk3 and mtor inhibition and WT controls. n = 15–17; log-rank test. (D and E) VCV (D) and percent FS (E) of the vmhcl^e13/e13 mutants after mapk3 and mtor inhibition compared to WT controls at 3 dpf. n = 7–8; data are presented as the mean ± SD; 1-way ANOVA.

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Acknowledgments

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