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Fig. 5 AFB1 induces nitric oxide production and arginine depletion in zebrafish larvae. (A) Representative fluorescence images of DAF-FM-DA stained 120 hpf larvae after embryonic exposure to AFB1. Fluorescence signals indicate nitric-oxide generation. Scale bar = 500 µm. The graphs showing the mean fluorescence intensities of (B) the whole larvae and (C) the abdominal region (yolk and intestinal area) of the larvae after AFB1 exposure (n = 2 replicates, n = 10 larvae per replicate). (D) Schematic representation of iNOS enzyme-catalyzed NO synthesis. (E)The graph showing the normalized free L-arginine levels of 120 hpf larvae after AFB1 exposure (n = 3 replicates, n = 30 pooled larvae per replicate). Data represent the mean and SD. “*” indicates statistical significance at p < 0.05 vs. the control (DMSO).
Reprinted from Journal of hazardous materials, 416, Ivanovics, B., Gazsi, G., Reining, M., Berta, I., Poliska, S., Toth, M., Domokos, A., Nagy, B., Staszny, A., Cserhati, M., Csosz, E., Bacsi, A., Csenki-Bakos, Z., Acs, A., Urbanyi, B., Czimmerer, Z., Embryonic exposure to low concentrations of aflatoxin B1 triggers global transcriptomic changes, defective yolk lipid mobilization, abnormal gastrointestinal tract development and inflammation in zebrafish, 125788, Copyright (2021) with permission from Elsevier. Full text @ J. Hazard. Mater.