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Figure 3 Short-term treatment with high DSF dose. Two-month-old mice were fed on standard or DSF-supplemented diet at an estimated dose of 200 mg/kg of body weight/day. (A) Body weight in animals fed on standard or DSF-supplemented diets for three months. Data are shown as mean ± SEM; n = 9 WT and 4 KO mice; ** p < 0.005, *** p < 0.0005 compared to untreated animals. (B) Cystine content in kidneys, liver, and heart of KO animals sacrificed at 5 months of age (n = 4 mice per group). Values are indicated as mean ± SEM; * p < 0.05 compared to untreated mice. (C) Representative immunohistochemistry images stained with anti-cleaved caspase-3 antibodies in kidneys obtained from WT (a,b) and KO (c,d) mice that were fed on standard (a,c) or DSF-supplemented (b,d) diets. Scale bar: 100 µm. The graph shows the mean cleaved caspase-3 intensity measured on five separate slides per animal (n = 3 mice per group).