ZFIN Image: Dalum et al., 2021, Figure 7

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Figure Caption

Figure 7

The ILT and ALT are structured by a complex network of reticulated epithelial cells. Representative deconvolved confocal images of adult zebrafish gills displaying the ILT (A) and the ALT (B) at high-magnification. The localization of sections (A, B) is illustrated on panels (C, D). The images were acquired from 30 μm whole-body cryosections stained with phalloidin (green) and DAPI (blue) and reticulated epithelial cells were labeled with an anti-cytokeratin antibody (red hot). While the cytokeratin signal is strong within the outermost layer of the epithelium, an intricate core of cytokeratin positive cells is observed within ILT (A) and ALT (B). Images are maximum intensity projections (2 µm). Annotations: Aa, Afferent artery; ALT, Amphibranchial Lymphoid Tissue; Bc, Branchial cavity; C, Cartilage; Ea, Efferent artery; Ga, Gill arch; ILT, Interbranchial Lymphoid Tissue; La, Lamella and S, Septum. Scale bars: 20 μm.

Acknowledgments

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