ZFIN Image: Ding et al., 2021, Figure 2

Image

Figure Caption

Figure 2

The blood clots are formed by RBC aggregation.

(A) The snapshot of Tg (gata1:dsRed) in smarca5^zko1049a and their siblings from 36 hpf to 2 dpf. The yellow rectangular boxes in CHT show the formed blood clots smarca5^zko1049a. (B) The bright-field of parabiosis at 2 dpf between smarca5^zko1049a and their siblings. The amplification region in the red rectangular box in CHT shows the blood clots (indicated by arrow heads) in smarca5^zko1049a and their siblings. (C) The confocal imaging of parabiosis generated between smarca5^zko1049a and their siblings with Tg (gata1:GFP) and Tg (gata1:dsRed) background, respectively. The magnification in CHT shows the aggregation of gata1:GFP⁺ cells (indicated by arrow heads) in the caudal vein plexus. (D) The confocal imaging of Tg (CD41:GFP) in smarca5^zko1049a and their siblings at 2 dpf. The magnification in the yellow rectangular box in CHT shows the blood clots and the distribution of CD41:GFP⁺ cells. (E) Drugs used to examine whether the blood clots in smarca5^zko1049a can be alleviated. (F) The bright-field of tail region in smarca5^zko1049a at 2 dpf in control group and with argatroban treatment. The blood clots are indicated by arrow heads. (G) The quantification of blood clots phenotype in (F). Data are mean ± s.d. (G). Asterisk presents statistical significance (**p< 0.01). p Values were calculated by two-tailed unpaired Student’s t-test.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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