ZFIN Image: Gui et al., 2021, Figure 1.

Image

Figure Caption

Figure 1.

The 48-nm repeat structure of bovine doublet microtubules (DMTs)

(A) Isolation of bovine DMTs for cryo-EM analysis.

(B) Two slices through the DMT map, showing density for the MIPs and ODA-DC. Protofilaments are numbered, and the seam of the A tubule is marked with an asterisk. MIP labeling continues in (C).

(C) The cross sections (top) show the DMT map colored by subunit, and the longitudinal sections (bottom) show the models of the MIPs, with tubulin in surface representation. Tektins are omitted for clarity in the longitudinal sections. PF, protofilament; IJ, inner junction.

In (B) and (C), the minus (−) and plus (+) ends of the DMT are indicated. See also Figures S1 and S2, Tables S1 and S2, and Video S1.

Acknowledgments

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Reprinted from Cell, 184(23), Gui, M., Farley, H., Anujan, P., Anderson, J.R., Maxwell, D.W., Whitchurch, J.B., Botsch, J.J., Qiu, T., Meleppattu, S., Singh, S.K., Zhang, Q., Thompson, J., Lucas, J.S., Bingle, C.D., Norris, D.P., Roy, S., Brown, A., De novo identification of mammalian ciliary motility proteins using cryo-EM, 5791-5806.e19, Copyright (2021) with permission from Elsevier. Full text @ Cell