ZFIN Image: Matrone et al., 2021, Fig. 4

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Figure Caption

Fig. 4

Effects of kdm1a knockdown in adult zebrafish.

A Western blotting (WB) analysis of Kdm1a control and morpholino KD. Dot plot shows semiquantitative analysis of bands. Two-tailed t-test. B Effects of Kdm1a KD on tailfin regeneration. Dashed red line represents the edge of the resection. Scale bar indicates 2 mm. C Line graph showing changes in tailfin regeneration rate following kdm1a KD. Two-way ANOVA followed by Bonferroni’s multiple comparisons test. D, E WB for H3K4unme (unmethylated), H3K4me1, H3K4me2, and H3K4me3 in control uninjured, injured and injured + kdm1a KD at 5 dpa. Dot plot shows semi-quantitative analysis of bands. Histone H3 was used as loading control. Dpa days post-amputation. Two-way ANOVA followed by Bonferroni’s multiple comparisons test, p values indicate comparisons of uninjured vs. other groups. N = 3 biological replicates. Data are presented as mean values +/− SEM.

Acknowledgments

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