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Fig. 8.
Treatment of Stk3-deficient mutant zebrafish for edema. None of the drugs in the study had obvious effects on edema onset in stk3+/+ controls. (A) Dose response of dexamethasone-induced early onset of the edema phenotype in stk3−/− larvae. The median times of onset of edema were 14.5 dpf (100 nM), 14.0 dpf (500 nM) and 13.5 dpf (1000 nM) compared to 15.0 dpf in DMSO control. Statistical analysis was performed using log-rank (Mantel–Cox) test (P<0.001). (B) Dose response of prednisolone-induced early onset of the edema phenotype in stk3−/− larvae. The median times of onset of edema were 14.0 dpf (10 μM), 13.5 dpf (50 μM) and 13.0 dpf (100 μM) compared to 15.0 dpf in DMSO control. Statistical analysis was performed using log-rank (Mantel–Cox) test (P<0.001). (C) Delay of edema onset by rapamycin treatment. The median times of onset of edema were 15.5 dpf (10 nM), 15.75 dpf (20 nM) and 16.5 dpf (50 nM) compared to 14.5 dpf in DMSO control. Statistical analysis was performed using log-rank (Mantel–Cox) test (P<0.05). (D) Delay of edema onset by tubastatin A treatment. The median onset points were 15.5 dpf (100 nM), 16.0 dpf (500 nM) and 18.5 dpf (1000 nM) compared to 15.0 dpf in DMSO control. Statistical analysis was performed using log-rank (Mantel–Cox) test (P<0.001).