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Fig. 2.
Functional redundance of yap1 and wwtr1 in pronephros development at embryonic stage. (A) Phenotype analysis of yap1−/−;wwtr1−/− embryos at 20 hpf. The embryos of yap1−/−;wwtr1−/− double mutant exhibited no tail extension. Scale bar: 0.5 mm. (B) Phenotype analysis of embryos with different genotypes produced by yap1+/−;wwtr1+/− in-cross. Scale bars: 1 mm (left) and 0.5 mm (right). (C) Whole-mount immunofluorescent staining of acetylated tubulin (red) and DAPI (blue) in yap1−/−;wwtr1+/− embryo and WT embryo at 48 hpf. Z projection of 0.5 μm optical section stacks. Scale bar: 10 μm.
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