Figure 5—figure supplement 2.

Figure 5—figure supplement 2.

(A) DMSO and (B) nocodazole-treated embryos (2.5 hr incubation from 17 to 19.5 hpf, as reported in Figure 5) hybridized with otx1-specific probe. (C) DMSO or (D) nocodazole-treated embryos with mitfa-specific probe. After nocodazole treatment, both mRNAs are specifically detected in the RPE (black arrowheads) despite its morphogenesis is impaired. (E, E’) Wild-type embryos labelled with laminin (green/basal), zo-1 (white/apical), and Hoechst (blue) after DMSO or (F, F’) nocodazole treatment. In both cases apico-basal polarity is maintained (white and green arrowheads), even after depolymerizing microtubules when RPE cells do not flatten correctly. A–D images are dorsal views of flat-mounted embryos. E–F’ images are frontal sections of one eye. Scale bars: 100 µm in A–D and 50 µm in E–F’.