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Figure 2
Treatment with BLD-2736 from 1 to 6 days post-fertilization reduces the presence of ataxin-3 protein species. (A) Representative scatterplots showing flow cytometric detection of detergent-insoluble EGFP-fused ataxin-3 particles following calpain inhibitor treatments. (B) The number of detergent-insoluble EGFP particles relative to nuclei was decreased following treatment with calpain inhibitors. (C) Representative western blot showing the presence of full length and cleaved ataxin-3 protein species. (D) Calpain inhibitor treatments were found to decrease the presence of full length ataxin-3 species in a dose-dependent manner. (E) Calpain inhibitor treatments also produced a decrease in the presence of cleaved ataxin-3 species, as detected by Western blotting. All data are presented as mean ± SEM. Each data point represents a single experimental replicate, where samples were pooled from n = 12–20 zebrafish larvae. * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001, **** represents p < 0.0001.