ZFIN Image: Rattka et al., 2021, Fig. 4

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Figure Caption

Fig. 4 (A) By screening for dysregulated genes via a cardiovascular disease assay we identified gap junction protein Connexin 43 (Cx43) to be downregulated (supplementary material Fig. A.3A) in Spen-deficient embryos 72 hpf, which was confirmed by cx43-specific qPCR in Spen-deficient hearts (0.47 ± 0.10, p = 0.0063, n = 3) as well as Spen-deficient embryos (0.46 ± 0.04, p = 0.0001, n = 3). (B) Western Blot analysis verifies downregulation of Cx43 in Spen-deficient embryos on the protein level. (C) Quantification of Cx43 levels in Western Blots of Spen-deficient and control embryos (n = 3). Images (B) have been cropped. A, C: Assessment of significance by t-test. *p < 0.05, **p < 0.01, ***p < 0.001.

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Acknowledgments

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Reprinted from Journal of Molecular and Cellular Cardiology, 155, Rattka, M., Westphal, S., Gahr, B.M., Just, S., Rottbauer, W., Spen deficiency interferes with Connexin 43 expression and leads to heart failure in zebrafish, 25-35, Copyright (2021) with permission from Elsevier. Full text @ J. Mol. Cell. Cardiol.