ZFIN Image: Zhao et al., 2021, Fig. 3

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Figure Caption

Fig. 3 Decreased HSPC proliferation and increased apoptosis in <italic>sart3</italic><sup><italic>smu471</italic></sup> mutants.

A, B Decreased HSPC proliferation in sart3^smu471 mutants. A The BrdU incorporation assay. The white arrowheads indicate proliferating HSPCs (cd41:eGFP^low; BrdU double positive cells). Green indicates eGFP, red indicates BrdU. B Statistical analysis of the percentage of CHT localized cd41^low HSPCs that incorporate BrdU (Student’s t-test, means ± SD; siblings, n = 28; sart3^smu471 mutants, n = 16; ****P < 0.0001). C, D Increased HSPC death in sart3^smu471 mutants. C The TUNEL assay. The white arrowheads indicate HSPCs undergoing cell death (cd41:eGFP^low; TUNEL double positive cells). Green indicates eGFP, red indicates TUNEL. D Statistical analysis of the percentage of CHT localized cd41^low HSPCs labeled by TUNEL (Mann–Whitney U test, means ± SD; siblings, n = 24; sart3^smu471 mutants, n = 27; *P < 0.05). Scale bars: 50 µm.

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Acknowledgments

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