FIGURE 5

FIGURE 5

Overexpression of wnt4 phenocopies zebrafish embryos injected with the COG4^p.G516G mRNA. (A) Graphs show the measured body length of each group after wnt4 mRNA injection at 3 and 7 dpf. The data are presented as mean ± SD. An unpaired two-tailed t-test was used. ^****p < 0.0001; ^∗∗p < 0.01; ^∗p < 0.05; ns, not significant. (B) Overexpression of wnt4 causes abnormal chondrocyte stacking and intercalation at Meckel’s cartilage. Ventral view of representative Meckel’s cartilage of zebrafish larvae after wnt4 injection at 4 dpf following WGA staining and imaged by a confocal microscope. (C) Overexpression of wnt4 causes cyclopia as expression of the COG4^p.G516G variant. Dorsal view of representative images of cyclopia compared to control. Two hundred picograms of wnt4 or COG4^p.G516G mRNA was used per embryo. Experiments were performed in triplicates with similar results.