ZFIN Image: Ruggiero et al., 2021, FIGURE 1

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Figure Caption

FIGURE 1

Above: Establishment of a TALEN-mediated per2 KO zebrafish line. A pair of TALENs was used to target the 2nd exon of the zebrafish per2 gene (TALEN left and right target sites are highlighted in yellow). A deletion of 8 bp (red dashes against a gray background) resulted in a frameshift mutation. Below: The consequent introduction of a premature stop codon and a predicted truncated PER2 protein of 160 aa compared with the 1399 aa WT protein. PER-ARNT-SIM domains (PAS, orange) and C-terminal of PAS domain (PAC, green) in the WT protein are indicated.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.