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Figure 5 FIGURE 5. Reactive oxygen species (ROS) production drives BCL2/adenovirus E1B 19kDa protein interacting protein 3 (Bnip3) expression in brain microvascular endothelial (bEnd.3) cells in response to hypoxia. (A) ROS analysis by flow cytometry and quantitative analysis of ROS intensity. (B) Western blot analysis of Bnip3. Mean±SD, n=3 independent experiments per group. *p<0.05 and **p<0.01. (C) Schematic illustrating the mechanism that Claudin-5 is involved in autophagy in cerebral vascular endothelial cells in the early stage of hypoxia.
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Full text @ Front. Physiol.