Figure 1
Structure and properties of Gran1: (A) Primary sequence and secondary structural elements of Granulysin. The cytolytic active site (amino acids 23–51) of Granulysin was synthesized and named “Gran1”. (B) Physicochemical properties of Gran1 were predicted using the Antimicrobial Peptide Databank server APD3 [18] for the molecular weight, amino acid composition, hydrophobic ratio, and Boman index. (C) Circular dichroism spectra of synthetic Gran1. The CD spectra were measured at decreasing pH by circular dichroism (n = 3). (D) Three-dimensional structure of Gran1 was measured. by 1H-based nuclear magnetic resonance and calculated with Ambiguous Restraints for Iterative Assignment (ARIA). The Left panel shows the 10 best fitting states for the Gran1 structure. The right panel represents the best fitting state of all ten measurements with the phi and psi angles closest in agreement with the Ramachandran plot.