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Figure 2
A qualitative food intake assay for zebrafish pomca mutant larvae. (A) Schematic representation of the feeding assay. Fluorescent intensities after free-feeding of 4-10-Di-ASP-labeled paramecia. Fluorescent intensities of ingested paramecia were at maximum levels 1.5 h after feeding (see Methods). (B) Side views of 7 dpf larvae examined under fluorescence illumination. The larvae were incubated with fluorescent microspheres coated with fish food for up to 1.5 h before visualizing the fluorescent contents in their gut. (C) Correlation between the relative amount of paramecia and fluorescent intensities of ingested paramecia in zebrafish. The fluorescent intensities were measured from the numbers of introduced paramecia thrice independently. All values are the mean ± SEM, n = 50.