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Figure 4 The influence of 6-GIN on the seizure-like behavior in the PTZ-induced zebrafish hyperlocomotion assay. After 24 h incubation in different doses of 6-GIN (12.5, 25, 31.25, or 37.5 µM), 7-day-old zebrafish larvae were exposed to PTZ (20 mM). Larval behavior was assessed 5 min after PTZ administration. Results of the assay are depicted as: (A) distance covered by larvae in 2 min long time bins, and (B) total distance covered by larvae during 30 min of the assay. Data were analyzed using one-way or two-way ANOVA with repeated measures followed by Tukey’s or Bonferroni’s post hoc test, respectively. Data are depicted as a mean ± SEM. Veh + veh (n = 48), 6-GIN 37.5 + Veh (n = 48), Veh + PTZ (n = 64), 6-GIN 12.5 + PTZ (n = 47), 6-GIN 25 + PTZ (n = 56), 6-GIN 31.25 + PTZ (n = 48), 6-GIN 37.5 + PTZ (n = 37). *** p < 0.001 vs. Veh + veh; &&& p < 0.001, & p < 0.05 vs. Veh + PTZ. 6-GIN—6-gingerol, PTZ—pentylenetetrazole, Veh—vehicle. n—refers to the total number of larvae.