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Figure 1 The influence of Zingiber officinale rhizoma methanolic extract on seizure-like behavior in the PTZ-induced hyperlocomotion assay in zebrafish. After 24 h incubation in Zingiber officinale rhizoma methanolic extract (60 µg/mL), 7-day-old zebrafish larvae were exposed to an acute dose of PTZ (20 mM). Larval behavior was assessed 5 min after PTZ application. Results of the experiment are depicted as: (A) distance covered by larvae in 2 min long time bins, and (B) total distance covered by larvae during 30 min of the assay. Data were analyzed using one-way or two-way ANOVA with repeated measures followed by Tukey’s or Bonferroni’s post hoc test, respectively. Data are depicted as a mean ± standard error of the mean (SEM). Veh + veh (n = 31), Ext 60 + veh (n = 24), Veh + PTZ (n = 48), Ext 60 + PTZ (n = 48). *** p < 0.001 vs. Veh + veh; &&& p < 0.001 vs. Veh + PTZ. Ext 60—Zingiber officinale methanolic extract (60 µg/mL), PTZ—pentylenetetrazole, Veh—vehicle. n—refers to the total number of larvae.