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Figure 2

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ZDB-IMAGE-210801-41
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Figures for Elsaey et al., 2021
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Figure 2 Analysis of human Atx1 allele expression in the stable transgenic zebrafish strains. Anti-HA immunostaining of HA-tagged proteins hAtx1[30Q] (A) and hAtx1[82Q] (B) in transgenic 4 dpf F2 larvae of established Atx1[30Q] and Atx1[82Q] strains crossed into the transgenic Tg(−7.5ca8:GFP)bz12 backgroundCytoplasmic and nuclear anti-HA-derived green fluorescence is surrounded by PC-specific membrane targeted red fluorescent GAPmScarlet expression (white arrows) The images display a dorsal view of the larval zebrafish corpus cerebelli, anterior is to the left. While in the upper row an overview over both cerebellar hemispheres is shown, the white boxes depict an area that was displayed at higher magnification in the lower row. Scale bars: 50 µm.

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