Figure 2

Figure 2

Levels of tfpia mRNA in a secondary knockdown screen of chromatin binding and regulatory proteins. Quantitative real-time PCR showing the fold change of tfpia gene expression in liver and spleen in knockdown of 9 chromatin binding and regulatory protein genes, brd7, brd9, ccdc61, ing2, ing3, ing4, supt16h, suz12a, and suz12b, with daily wild-type (WT) controls using one-way ANOVA. The bar graphs represent daily wild-type controls (squared bars), and all knockdowns (open bars and dotted bars) to the right of each wild-type were performed on the same day. Error bars represent mean ± SD. Six fish were used for each of the chromatin binding and regulatory protein gene knockdown and control experiments (N = 6). The lines on the top represent a significant difference between wild-type and knockdown samples. *, **, and *** represent p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001, respectively. p-value < 0.05 was considered significant.