ZFIN Image: Jamalpoor et al., 2021, Figure EV1

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Figure Caption

Figure EV1 Generation of <italic>CTNS</italic><sup>−/−</sup> isogenic cell line of ciPTEC using CRISPR

Schematic overview of the CRISPR‐based strategy to knockout the CTNS gene in ciPTEC.

Sanger sequencing chromatogram shows resulting sequence in CRISPR‐generated cystinotic cells (CTNS^−/−).

Quantification of cystine levels (nmol/mg protein) by HPLC‐MS/MS in control (CTNS^WT), CRISPR‐generated cystinotic cells (CTNS^−/−; line 3, 7 and 35, and patient‐derived cystinotic cells (CTNS^Patient) (n = 3–6).

Quantification of cystine levels (nmol/mg protein) by HPLC‐MS/MS in CTNS^−/− lines (3, 7 and 35), and CTNS^Patient cells upon treatment with cysteamine (100 µM) (n = 3–6).

Data information: Data are expressed as mean ± SEM. *P‐values < 0.05 were considered to be significant. One‐way ANOVA with Dunnett’s correction (C and D) or unpaired t‐test (D). Exact P‐values and statistical tests are listed in Appendix Table S1.

Acknowledgments

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