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Figure 1 Activation of BMP signaling pathway during ventricle regeneration. (A-D') Whole-mount in situ hybridizations showed upregulated expression of BMP signaling components in ablated hearts (A'-D') compared to control hearts (A-D) at 5 dpf/2 dpt. (E-G) Confocal stack projections of Tg(vmhc:mCherry-NTR; Bre:dGFP) hearts showed BMP signaling activation at 1-2 dpt. (H-J) Confocal stack projections of immunofluorescence showed BMP signaling activation at 1-2 dpt. Green, phospho-Smad1/5/9; red, myosin heavy chain (MF20). (K-N') Overlay of Bre:dGFP and pSmad1/5/9 signals in myocardium and epicardium at 2 dpt. (K-N) stack projections, (K'-N') optical sections. Scale bars, (A-D') 25 µm, (E-N') 50 µm. dpf, days post fertilization; dpt, days post MTZ-treatment; A, atrium; V, ventricle. Dashed lines outline the hearts.