ZFIN Image: Mikdache et al., 2021, Figure 7

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Figure Caption

Figure 7

Loss of rgs4 function alters mTOR signaling in neural cells (A) Immunoblotting of lysates from zebrafish embryos at 20 hpf. S6 and p-S6 amounts were normalized to α-tubulin and the ratio of p-S6 relative to S6 was compared between WT and MZrgs4 lysates. No significant difference was observed between the two groups. Immunoblotting of lysates from zebrafish embryos at 48 hpf. S6 and p-S6 amounts were normalized to β-actin and the ratio of p-S6 relative to S6 was compared between WT and MZrgs4 lysates. MZrgs4 embryos show a significant decrease in the amount of p-S6/S6 in comparison to WT. (B) Immunoblotting of neural lysates from zebrafish embryos at 48 hpf. S6 and p-S6 amounts were normalized to β-actin and the ratio of p-S6 relative to S6 was compared between WT and MZrgs4 lysates. MZrgs4 embryos showed a significant decrease in the amount of p-S6/S6 in comparison to WT.

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Acknowledgments

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