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FIGURE 2
Cells were subjected to immunofluorescence analysis using an antibody directed against AIF1 (green). Nuclei were counterstained with DAPI (blue). The fluorescence was visualized and imaged under a fluorescence microscope. C: control group; M: morphine group; M + M: morphine+methadone group; M + I: morphine+isorhynchophylline; M + MH: morphine+minocycline hydrochloride.
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