ZFIN Image: Lu et al., 2021, FIGURE 3

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Figure Caption

FIGURE 3

Phenotypes of zebrafish embryos with overexpression of DSCAM and bace2. Zebrafish embryos were injected with 100 pg of DSCAM mRNA per embryo and 100 pg of bace2 mRNA per embryo, respectively. (A) The expression levels of dscama, dscamb, and bace2 of mRNA injected embryos were analyzed by qRT-PCR at 48 hpf (^∗P < 0.05, ^∗∗P < 0.01). (B) No significant difference was found in the neuron numbers of the last six somite lengths of gut between control and mRNA injected embryos at 5 dpf. The HuC/D antibody was used to stain the enteric nervous system (ENS) neurons, and the neuron numbers in the last six somite lengths of gut were counted. Embryos injected with DSCAM mRNA, n = 67. Embryos injected with bace2 RNA, n = 53. Control embryos, n = 28. (C) The overexpression of DSCAM and bace2 caused no obvious defect of enteric neurons in the hindgut.

Acknowledgments

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