FIGURE 3

FIGURE 3

mbp⁺ membrane changes are observed following exposure to lysolecithin. All images are lateral views of the spinal cord with anterior to the left and dorsal to the top. (A,B) Expression of mbp:egfp-CAAX appears as sheath-like structures in the dorsal and ventral spinal cords in 4 (A) and 6 dpf (B) zebrafish larvae. (C) Following injection of a control solution into the spinal cord of a 4 dpf mbp:eegfp-CAAX larva, the mbp⁺ membrane sheaths remain unchanged throughout the duration of the time-lapse movie. (D) Following injection of a lysolecithin (experimental) solution into the spinal cord of a 4 dpf mbp:egfp-CAAX larva, the mbp⁺ sheaths dynamically change to form ovoids (arrowheads). (E) Quantification of the percentage of zebrafish with mbp:egfp-CAAX membrane changes at 8 hpi. p < 0.0001; n = 6 control; n = 5 experimental. (F) Quantification of the average number of mbp:egfp-CAAX ovoids observed at 4, 6, and 8 hpi demonstrates an increase in the number of ovoids within the lysolecithin (experimental) group (n = 6) when compared with the control group (n = 6). At 6 hpi, *p = 0.0493. (G) Mosaically labeled oligodendrocytes by injection of mbp:mcherry-CAAX. Larvae injected with a control solution (control, top) or lysolecithin (exp. group, bottom) at 6 dpf. Following injection of a control solution (top), the oligodendrocyte remains relatively unchanged throughout the course of the time-lapse movie. In contrast, an ovoid-like structure is observed within the mbp:mcherry-CAAX⁺ oligodendrocyte beginning around 8.1 hpi. Red dashed lines denote the spinal cord. Scale bars, 25 μm.