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Figure 1 - figure supplement 1

ID: ZDB-IMAGE-210607-30
Source: Figures for Li et al., 2021

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Figure Caption

Figure 1 - figure supplement 1 <italic toggle='yes'>ccm2</italic> CRISPR zebrafish exhibit mosaic expression of CCM2.

(A) Outline of the experiment: cas9 mRNA and gRNAs targeting ccm2 were co-injected into one-cell stage zebrafish embryos. (B) Sequencing showed that the Cas9 together with designed gRNA could successfully target ccm2 and produce indels. PAM sequence (5’-AGG-3’) is indicated in a rectangle. Though the wild-type sequence is the top read in each downstream position, indicating existence of wild-type allele, multiple reads emerged downstream of the PAM in ccm2 CRISPR embryos. (C) Whole mount in situ hybridization of 2 days post fertilization (dpf) embryos showed ccm2 CRISPR embryos displaying patchy loss of CCM2 expression.

Acknowledgments

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