ZFIN Image: Okuda et al., 2021, Figure 7.

Image

Figure Caption

Figure 7. A two-step mechanism for activating and maintaining Erk activity in regenerating vessels.

Schematic representation of the two-step mechanism employed by endothelial cells (ECs) to activate Erk-signalling following vessel wounding. Pre-ablation (left), the majority of ECs are Erk-signalling inactive. Following vessel wounding (middle), both ablated and adjacent intersegmental vessel (ISV) ECs rapidly activate Erk-signalling. Ca²⁺ signalling is also rapidly activated following vessel wounding but only in ablated ISV ECs, particularly in ECs close to the wounded site. Ca²⁺ signalling activity contributes to the activation of Erk-signalling in ablated ISV ECs in a sequential manner, starting from ECs close to the wounded site. Erk-signalling in adjacent ISV ECs has returned to pre-wound levels by 3 hours post-ablation (hpa) (right). Erk activity in ablated vessel ISV ECs is sustained through Vegfr-signalling. ECs closer to the wounded site are less sensitive to Vegfr-signalling inhibition, with higher signalling compared to ECs farther away. Recruited macrophages are essential for vessel regeneration but not the sole source of Vegfs at 3 hpa.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife