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Fig. 1 (A) EIF4A3/Eif4a3 interacts with β-catenin, as indicated by co-immunoprecipitation. Upper panel: exogenous EIF4A3 interacts with β-catenin. Middle panel: endogenous β-catenin and EIF4A3 interact with each other in HEK293T cells. Lower panel: endogenous β-catenin and Eif4a3 interact with each other in zebrafish embryos at 24 hpf. (B,C) Determination of the region of EIF4A3 required for its interaction with β-catenin. Schematic diagram of human EIF4A3 protein domains are shown in B. Various Flag-tagged EIF4A3 mutants were co-expressed with Myc-tagged β-catenin in HEK293T cells and cell lysates were subjected to co-immunoprecipitation (C). (D,E) Determination of the region of β-catenin required for the EIF4A3 interaction. Schematic diagram of human β-catenin protein domains is shown in D. Various Myc-tagged β-catenin truncations were co-expressed with Flag-tagged EIF4A3 in HEK293T cells and cell lysates were subjected to co-immunoprecipitation (E).