Fig. 2

Fig. 2 (A) Sequencing chromatographs of control npc2, npc2^y536 and npc2^y537 alleles. Arrowheads indicate the area of the induced deletion and nucleotide positions corresponding to the wild-type npc2 gene sequence. (B) Genotyping of npc2^y536 was carried out by using a derived cleaved amplified polymorphic sequence (dCAPS) primer to introduce an artificial BglII restriction enzyme site in the PCR product amplified from the wild-type npc2 allele, followed by BglII digest. The PCR product from control but not npc2^y536 was expected to be cut by BglII. (C) A dCAPS primer was used to introduce an artificial HpyCH4IV restriction enzyme site in the PCR product amplified from the control npc2 allele, followed by HpyCH4IV digest. The PCR product from control but not npc2^y537 was expected to be cut by HpyCH4IV.