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Fig. 1 (A) mRNA expression of npc2 was characterized by in situ hybridization. Expression was ubiquitous at the 4-cell and sphere stage and became more intense in the yolk syncytial layer (arrowheads) to up to 3 dpf. Number of embryos showing representative staining over total embryos examined are indicated in each image. Scale bars: 200 μm (4-cell, Sphere and Shield); 500 μm (24 hpf, 30 hpf and 3 dpf). (B) RT-PCR assessment of npc2 and npc1 mRNA expression from the 4-cell stage to 72 hpf. gapdh was used as an internal control.
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