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FIGURE 3 Evaluation of gonadal tissue and plasma androgen levels of WT and stra8 crispant salmon males. (A,C) Gonado-somatic indices (GSI) and plasma androgen (11-ketotestosterone; 11-KT) levels in WT and stra8 crispant males at samplings from May (maturing) and September 2017 (mature). (B,D) Histological images of maturing (B) and mature (D) Atlantic salmon testes obtained from WT and stra8 crispant fish. Insets show testis tissue magnified from the marked area (black dashed line). White arrowheads indicate apoptotic germ cells. Representative germ cell stages are shown in the upper right panels: type A spermatogonia (SPG A), type B spermatogonia (SPG B), spermatocytes (SPC), round spermatids (rSPT), elongated spermatids (eSPT) and spermatozoa (SPZ). WT, wild-type; Crispant, stra8 crispant. Scale bar, 50 μm.