Fig. 1

Fig. 1 TiO2 NPs size distribution in medium and level in larvae. Zebrafish embryos were exposed to 0.1 mg/L TiO₂ NP and TiO₂ NP with 2 µM TBPPA mixture from 8 to 120 hpf and exposure medium and larvae were sampled for TiO₂ analysis. (A) Particle size analysis of exposure medium collected at 24 h after treatment. (B) TiO₂ level in the medium collected at 24 h after treatment (n = 3) and exposed larvae at 120 hpf (n = 3, 20 larvae per replicate). Values plotted are mean ± SEM and *P < 0.05 using unpaired t-test analysis.