Fig. 1 RA signaling promotes specification of posterior nkx2.5+ progenitors. (A–F) ISH for nkx2.5:Kaede (Kaede) in DMSO-treated (control) and DEAB-treated embryos at the 14s, 18s, and 20s stages (16, 18, and 19 hpf). Brackets in A and B indicate the length and width of the nkx2.5:Kaede expression. Nkx2.5 expression is expanded laterally and posteriorly and the posterior pharyngeal artery progenitors are not a distinct aggregate in RA deficient embryos. Views are dorsal with anterior up. Arrows in C and E indicate the space between the anterior nkx2.5+ and posterior nkx2.5+ aggregates. Arrows in D and F indicate the posterior limit of nkx2.5 expression and lack of posterior nkx2.5 aggregates. n = 25 for all conditions examined.
Reprinted from Developmental Biology, 473, Duong, T.B., Holowiecki, A., Waxman, J.S., Retinoic acid signaling restricts the size of the first heart field within the anterior lateral plate mesoderm, 119-129, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.