Fig. 1

Fig. 1 Fig. 1. Cardiomyocyte cell cycle activity drops to low levels prior to complete morphological regeneration. (A) The fraction of PCNA+ EGFP+ cardiomyocytes in myl7:EGFP^twu34 fish within 150 ​μm of the wound border peaks at 7 dpi and drops to levels barely above baseline by 30 dpi. uninj, uninjured. Error bars represent CI 95%. n ​= ​5 hearts for each group. One-way ANOVA ​+ ​Dunnett’s multiple comparisons test. Numbers indicate p-value. (B) Fraction of EdU⁺ EGFP⁺ cardiomyocytes in myl7:EGFP^twu34 fish. For each time point, a single intraperitoneal injection of EdU was performed 24 ​h prior to heart extraction. n (hearts) ​= ​5 (uninjured), 5 (3 dpi), 5 (7 dpi), 7 (14 dpi), 8 (30 dpi), 7 (60 dpi). One-way ANOVA ​+ ​Dunnett’s multiple comparisons test. (C) Many hearts contain wound tissue detected by Acid Fuchsin Orange G (AFOG) staining at 30 dpi. Red: fibrin, blue: collagen, brown: myocardium. Scale bars, 100 ​μm. (D) Percentage of hearts without residual wounds (= 0% of ventricle area), small wounds (between 0% and 3%) or bigger wounds (>3%) at 14, 30 and 90 dpi. n ​= ​31 (14 dpi), 29 (30 dpi), 58 (90 dpi). Chi-square test.