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Fig. 1 kctd15a and kctd15b are expressed in developing distal nephron precursors. (A) Whole-mount in situ hybridization of kctd15a and kctd15b during wild-type embryogenesis. Black arrowheads indicate expression within the developing renal field. Scale bar: 200 μm. (B) Whole-mount in situ hybridization of kctd15a and kctd15b during wild-type embryogenesis. Black arrowheads indicate expression within distal nephron precursors. Scale bar: 200 μm. (C) Fluorescent in situ hybridization of kctd15a (magenta) and tfap2a (green) in the 22 ss wild-type distal nephron. White rectangle outlines the region in the insets. Scale bars: 5 μm (insets); 35 μm (main image). (D,E) Fluorescent in situ hybridization of tfap2a (green), kctd15a (magenta in D) and kctd15b (magenta in E) at 24 hpf in the pronephros. White rectangle outlines the region shown at higher magnification (bottom panels). Cyan outline indicates an example of a co-expressing cell. Scale bars: 35 μm (top); 5 μm (bottom). Yellow arrowheads indicate intense areas of transcript co-expression in C-E.