ZFIN Image: Banks et al., 2021, Fig. 7

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Figure Caption

Fig. 7 Figure 7. Socs3b mRNA Is Hypermethylated in tet2/3^DM Neutrophils (A) RNA methylation status from RNA bisulfite-sequencing data for each CpG site in the socs3b locus for sorted wild-type or tet2/3^DM 2-dpf neutrophils; n = 10/condition. Red triangles indicate cytosines mutated in the differentially methylated cytosine (DMC) mutant mRNA used in the following experiment. (B) Relative levels of socs3b mRNA at 1–4 dpf assessed by qRT-PCR that measures levels of the injected wild-type or DMC mutant transcripts; n = 6 groups of 5 embryos per data point, ∗p < 0.05 by 2-way ANOVA with Tukey post hoc test. (C) Minimum free energy RNA structure of socs3b. DMCs are highlighted by triangles. Red triangles represent bases that were mutated in generating the DMC mutant socs3b mRNA and black triangles represent unedited bases. Inset: a close-up of an RNA arm with several DMCs closely packed together indicated by the red box in the full structure.

Acknowledgments

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