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Fig. 6 Fig. 6. Cortisol-mediated [(Ca2+)i] rise involves calcium release-activated calcium channel (CRACC) activation. (a) Representative images of the developing trunk muscle (DTM) loaded with the ratiometric dye FURA-2AM and treated with either vehicle (CONTROL), cortisol (CORT) without or with antibody for quenching (CORT + Ab), and CRACC inhibitor (CpD5J-4) without or with cortisol (CpD5J-4+CORT). (b) A bar graph showing the ratiometric intensity at 30 s with the CRACC inhibitor CpD5J-4 either with or without cortisol. (c) A bar graph showing the ratiometric intensity at 30 s with CORT either without or with the antibody (CORT + Ab). All bars represent mean ± SEM (n = 4–5 independent embryos); bars with different letters are significantly different; one way ANOVA; Holm Sidak post hoc test; p < 0.05.
Reprinted from Molecular and Cellular Endocrinology, 520, Das, C., Faught, E., Vijayan, M.M., Cortisol rapidly stimulates calcium waves in the developing trunk muscle of zebrafish, 111067, Copyright (2020) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.