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Figure 6—figure supplement 2. <italic>TgBAC(vegfd:</italic>EGFP) and <italic>TgBAC(vegfc:</italic>EGFP) expression patterns in WT and <italic xmlns:xlink='http://www.w3.org/1999/xlink'>vegfab</italic> mutants which displayed the presence or absence of the DLV.

(A–C") Dorsal views of 54 hpf vegfab+/+ (A–A") and vegfab-/- (B–C") heads of embryos that carried the TgBAC(vegd:EGFP) and Tg(kdrl:ras-mCherry) transgenes. vegfab-/- embryos that exhibited the presence (B–B"), or absence (C–C"), of the DLV were examined. Overall TgBAC(vegd:EGFP) expression patterns and signal intensity did not apparently differ between vegfab+/+ (A’, n = 14) and vegfab-/- embryos regardless of the presence (B’, n = 7), or absence (C’, n = 4), of the DLV. (D–F") Dorsal views of 48 hpf vegfab+/+ (D–D") and vegfab-/- (E–F") heads of embryos that carried the TgBAC(vegc:EGFP) and Tg(kdrl:ras-mCherry) transgenes. vegfab-/- embryos that exhibited the presence (E–E"), or absence (F–F"), of the DLV were examined. Overall TgBAC(vegc:EGFP) expression patterns and signal intensity did not apparently differ between vegfab+/+ (D’, n = 3) and vegfab-/- embryos regardless of the presence (E’, n = 2), or absence (F’, n = 3), of the DLV. Scale bars: 50 μm.

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