Figure 1

Figure 1

Tail transection in zebrafish larvae as a model for inflammation. (A) Schematic drawing of a zebrafish larva at 3 dpf. The dashed red line shows a site of transection (in some studies, the transection site may not include the a part of notochord). (B–E) Confocal microscopy images of tail from amputated larvae of the following transgenic lines: Tg(mpeg1:mcherry-F)(B), Tg(mpx:GFP)(C), Tg(il1b:GFP)(D), Tg(mpeg1:mCherry-F/tnfa:eGFP-F)(E). Images were taken at 4 h post-amputation using a Nikon Eclipse Ti-E microscope with a Plan Apo 20X/0.75 NA objective. Images show accumulation of macrophage (B) and neutrophils (C), activation of the il1b gene (D), and tnfa expression in macrophage (E) near the wound. In (E), arrow heads indicate macrophages in which tnfa was activated.