ZFIN Image: Fish et al., 2021, Figure 3

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Figure Caption

Figure 3 Genome editing of fga exon 2. (A) Aligned N-terminal region of human and zebrafish fibrinogen Aα chains. Aα R35 and Aα R28 are indicated. (B) CRISPR/Cas9 strategy for fga exon 2 editing through homology directed repair (HDR). In red is part of the sgRNA targeting the edited region by complementarity, in bold is the PAM sequence. Part of the ssODN for HDR is shown to highlight edited nucleotides (blue), including codon usage changes and the Arg to Cys codon change. (C) Sanger sequencing to confirm genome edited sequence in genomic DNA from homozygous mutant fish (fga^mut/mut), compared to wild-type (fga^+/+). Changed nucleotides are highlighted with arrows.

Acknowledgments

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