ZFIN Image: Khan et al., 2020, FIGURE 4

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FIGURE 4

Carbofuran accelerated autolysosomal puncta formation in a double‐transgenic zebrafish line of spns1 mutant background, expressing EGFP‐LC3 and mCherry‐Lamp1. A, Carbofuran exposure accelerated both autophagosomal EGFP‐LC3 and lysosomal mCherry‐Lamp1 expressions in spns1 mutant zebrafish. Lysosomal mCherry‐Lamp1 expression was merged to EGFP‐LC3 expression (bottom row; yellow colour). B, Quantification of autophagosomal EGFP‐LC3 expression. C, Quantification of lysosomal mCherry‐Lamp1 expression. D, Quantification of the co‐localization of expressions. Both EGFP‐LC3 and mCherry‐Lamp1 expressions (with their co‐localization) were significantly increased by the loss of the spns1 gene and were further significantly up‐regulated by carbofuran exposure. The scale bar is 10 mm; n = 6; *P ≤ .05; **P ≤ .01; ***P ≤ .005

Acknowledgments

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