ZFIN Image: Carroll et al., 2020, Fig. 7.

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Figure Caption

Fig. 7.

ANC of esrp1/2 double mutants is populated by undifferentiated cells. Representative z-stacks of RNAscope ISH of coronal sections of esrp1/2 double CRISPR mutants and WT clutch-mate controls at 4 dpf. (A) Sections through ANC anterior to the eyes. col2a1 (red) staining depicts normal morphology of the ANC cartilage elements in WT, while a cleft is apparent in the esrp1/2^−/− zebrafish, with dapi (blue)-stained cells between adjacent trabeculae (arrowheads). These col2a1^– cells do not express epithelial markers krt4 (cyan) or krt5 (magenta), except around the periphery. (B) Sections posterior to those in A show col2a1^– cells continuing inferior to the trabeculae in the esrp1/2 mutant zebrafish, and cells have low expression of irf6 (boxed area). (C) Zoomed image of col2a1^– cells from the boxed area in B, showing irf6 expression (green). Dashed lines outline ANC cartilage elements. Scale bars: 50 μm.

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Acknowledgments

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