ZFIN Image: Carroll et al., 2020, Fig. 8.

Image

Figure Caption

Fig. 8.

Aberrant ANC cells of esrp1/2 double mutants express CNCC and epithelial cell markers. Representative z-stacks of RNAscope ISH of coronal sections of esrp1/2 double CRISPR mutants and WT clutch-mate controls at 4 dpf. (A) Sections through the ANC anterior to the eyes. (B) Medial sagittal sections through the ANC (anterior to left). Dashed lines outline the ANC cartilage elements. col1a1 (white) staining depicts perichondrium surrounding the aberrant mass of cells in the esrp1/2 mutant zebrafish, consistent with chondrogenic condensation (leftmost arrowhead). irf6 (green) and sox10 (red) expression is apparent in these cells (indicated by arrowheads in respective columns); dapi is shown in blue. Scale bars: 20 μm.

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Acknowledgments

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