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FIGURE 8
Knockdown of ASGR and HNF4A reverses the effect of oligo‐fucoidan reduced the hepatoma cell viability. A, Oligo‐fucoidan reduced the cell viability of three hepatoma cells. B, Western blot analysis indicating that knockdown of ASGR by sh‐ASGR1‐1 and sh‐ASGR1‐2 reduced the ASGR protein level and pSTAT3. Cell viability assay demonstrated that OF treated for 48 hours significantly reduced cell proliferation and sh‐ASGR1‐1 and sh‐ASGR1‐2 significantly reversed the OF effect. C, Knockdown of HNF4A by sh‐HNF4A reverse the OF inhibiting hepatoma cell viability. D, STAT3 inhibitor synergize with oligo‐fucoidan on reduction of hepatoma cell viability.