ZFIN Image: Lee et al., 2020, Figure 1

Image

Figure Caption

Figure 1—figure supplement 1. Projections of NPVF neurons to the serotonergic IRa shown in single optical sections.

(A,B) Serial optical sections (0.5 μm thick) in the hindbrain of a 6-dpf Tg(npvf:eGFP) animal labeled with a 5-HT-specific antibody (magenta), which were used to generate the image shown in Figure 1K. Fibers from npvf-expressing neurons (green) do not innervate SRa soma (A) but do innervate IRa soma (B). (C) A 4-μm-thick optical section of a brain from a 6-dpf Tg(npvf:KalTA4); Tg(UAS:nfsb-mCherry); Tg(tph2:eNTR-mYFP) animal. White bracket indicates the IRa and is magnified in panels (D,E), which show 0.6-μm-thick serial optical sections. Fibers from npvf-expressing neurons (magenta) do not innervate SRa neurons (green, D) but do innervate IRa neurons and their fibers (green, E). Hyp, hypothalamus, IRa, inferior raphe; D, dorsal; V, ventral; A, anterior; P, posterior. Every third section (A,D) and every other section (B,E) is shown. Scale: 50 μm (C), 10 μm (B,E).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife