ZFIN Image: Chetty et al., 2020, Fig. 6

Image

Figure Caption

Fig. 6 Ets1 activity depends on MAPK signaling and phosphorylation of conserved Thr30 and Ser33 residues. (A–F) Confocal micrographs of 12-somite stage Tg(fli1a:GFP) wild-type, XeX:etv2 and XeX:ets1-injected embryos treated with either DMSO (A–C) or 15 μM MAPK inhibitor SL327 (D–F) from 70% epiboly-12-somite stage. Note the absence of ectopic GFP fluorescence in XeX:ets1 embryos treated with SL327 (F) compared to those treated with DMSO (C). (G–I) Confocal micrographs of 15-somite stage Tg(fli1a:GFP) wild-type embryos, XeX:ets1 and XeX:pMut-ets1 (containing Thr30Ala and Ser33Ala mutations) embryos. Note the reduction of ectopic GFP expression in embryos injected with the XeX:pMut-ets1 construct containing mutated phosphorylation sites (I) compared to XeX:ets1-injected embryos (H). White arrowheads indicate ectopic GFP expression.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 465(1), Chetty, S.C., Sumanas, S., Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish, 11-22, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.