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Figure 4

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ZDB-IMAGE-200820-6
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Figures for Bryant et al., 2020
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Figure 4

Constitutive homozygous snx14 mutations do not impact zebrafish morphology at 4dpf but do increase FAs from neutral lipid and phospholipids. (A) Illustration and demonstration of zebrafish eye (E) width and head (H) width measurements of maternal zygotic (MZ) snx14Mut/Mut fish derived from snx14Mut/Mut female and snx14WT/Mut male pairs. (B) Maximum projected confocal images of heads (dorsal view) from 4dpf zebrafish embryos either snx14WT/Mut or MZ-snx14Mut/Mut. Staining employed immunohistochemistry against acetylated tubulin (green), marking axon tracts and SV2 (magenta) marking neuropil areas. (CE) Relative FA levels from whole body lysates of 4dpf zebrafish. Neutral lipid fraction-derived FA 16:0 (C), FA 18:1(n9) (D) and FA 20:4(n6) (E) were elevated in snx14Mut/Mut zebrafish compared to both snx14WT/WT and snx14WT/Mut zebrafish. (FH) Phospholipid fraction-derived FA 16:0 (F), FA 18:1(n9) (G) and FA 20:4(n6) (H) were elevated in both snx14Mut/Mut and snx14WT/Mut zebrafish compared to snx14WT/WT zebrafish. N = 3 (Pool of 6 zebrafish in each lysate), circles = individual lysate values, bars = mean, error bars = SD, **(p ≤ 0.01), n.s. (p ≥ 0.05), one-way ANOVA.

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